Evaluation of Antibacterial Activities of Environmental Benign Synthesis of Silver Nanoparticles using the Flower Extracts of Plumeria Alba Linn.

Abstract

Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. However, development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study green synthesis of stable silver nanoparticle was done using the flower extract of Frangipani (Plumeria alba). The formation of silver nanoparticles was apparently displayed within 2 min with evidence of surface plasmon bands (SPB) between  440–460 nm  and  characteristic  color  changes  from  pale  yellow  to  reddish brown. These biosynthesized nanoparticles were characterized with the help of UV–vis spectrophotometer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bio reduced silver nanoparticles was analyzed using UV–vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and gram-positive microorganisms using gentamicin as control. It was observed that P. alba flower extract can reduce silver ions into silver nanoparticles within a minute of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size average diameter of 12.4 nm possessing antibacterial activity. From the results, there is evidence of inhibition towards bacterial growth. It is suggested that biosynthesis of silver nanoparticles could be a gateway to our numerous health issues.

Keywords:  Plumeria alba, silver nanoparticles, gentamicin, antibacterial activity