Induction of Specific Cytotoxic Lymphocytes in Goats Vaccinated with Fractionated Antigens of Brucella melitensis 16M

Abstract

The present study investigated the ability of different fractionated antigens of Brucella melitensis 16M to induce cytotoxic T-lymphocytes. For this study, soluble Lipopolysaccharide and outer membrane protein antigens were fractionated by using gel filtration chromatography and found 6, 5 and 5 fractions respectively of each antigen. All the regions of antigens were analyzed for their ability to induce cytotoxic T-lymphocytes. This study investigated that SAG5, LPG1 and OMPG4 induce maximum cytotoxicity, and after comparing these three immunoreactive fractions, it was found that LPG1 induces maximum CTLs followed by OPMG4 and SAG5 respectively.

Keywords: B. melitensis, cytotoxic T- lymphocytes, cytoxicity, fractionated antigens