Hepatoprotective Efficiency of Nigella sativa L. Extract on D-Galactosamine Induced Toxicity

Abstract

The Protective role of Nigella Sativa L. alcoholic extract (NSE) was investigated on D-Galactosamine (D-GalN) induced hepatotoxicity in rats. Twenty four adult wistar albino rats were divided into four groups viz Control, D-Galatosamine induced hepatotoxicity, Nigella sativa alcoholic extract and Nigella Sativa alcoholic extract pretreatment prior to the administration of D-Galactosamine. After treatment, the rats were sacrificed, blood  and liver samples were collected for determination of Serum Aspartate transaminase (AST), Alanine trasnaminase (ALT), and Alkaline phosphatase (ALP) and liver lipid Peroxidation(LPO), Reduced glutathione (GSH), Glutathione peroxidase (GPx),  Catalase (CAT) and Super oxide dismutase (SOD) activity. Mean AST, ALT, ALP and LPO levels were found to be significantly higher in D-GalN induced hepatotoxic rats group (P<0.05) than in the control group. Mean GSH, GPx, CAT and SOD levels were significantly lower in liver tissue of D-GalN administered group compared (P<0.05) with the control group. Levels of these parameters were found to be nearer to control in the NSE pretreated group. Results of the present study strongly indicate that NSE having hepatoprotective efficiency againt D-GalN induced toxicity in rats.