Production and Characterization of Biosurfactant from Indigenous Bacterium Bacillus amyloliquefaciens USTBb: Feasibility in Microbial Enhanced Oil Recovery

Autoři

  • Radhika Chandankere University of Science and Technology Beijing, Xueyuan Road No.30, Haidian District, Beijing
  • Jun Yao
  • Kanaji Masakorala
  • A.K Jain
  • Ranjan Kumar

Klíčová slova:

biosurfactant, surface tension, crude oil, microbial enhanced oil recovery, stability, characterization

Abstrakt

In the present study, the production and characterization of biosurfactant by an indigenous Bacillus methylotrophicus USTBb (GenBank accession no.KF496216) was investigated. This bacterial strain was isolated from crude oil contaminated aqueous medium was identified to be a persuasive producer of biosurfactant using crude oil as the sole carbon and energy source. Growth parameters were optimized, with the aim of increasing biosurfactant productivity. Maximum biosurfactant production (5.5 g L-1) by strain USTBb was reached after 12 d of incubation time and was found to be growth-associated. The strain was able to reduce the surface tension of its cell free culture supernatant containing biosurfactant to 28 mN m-1. Biosurfactant exhibited excellent emulsification activity against crude oil when compared with various tested hydrocarbon and vegetable oil and it possessed a low critical micelle concentration (CMC) value of approximately            35        mg L-1, indicating high surface activity. USTBb biosurfactant showed stability under extreme environmental conditions such as high temperature, pH and salinity. Elemental compositions and thermal analysis of biosurfactant was determined by X-ray photoelectron spectroscopy (XPS) and thermal gravimetric (TG) respectively. Furthermore, biosurfactant was found to be an antimicrobial agent against gram positive and Gram negative bacterial strains. Studies on the crude oil recovery using biosurfactant produced by Bacillus sp. USTBb suggested its prospective relevance in microbial enhanced oil recovery (MEOR) and bioremediation.Keywords: biosurfactant; surface tension; crude oil; microbial enhanced oil recovery; stability; characterization

Stažení

Publikováno

2014-05-08

Číslo

Vol 4 No 1 (2014)

Sekce

Research Articles

Licence

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

·         I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.

·         I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).

·         I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.

·          I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

_______________

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.