Cytotoxicity of Titanium Dioxide Nanopaprticleson A Bacterial Population Isolated from Lakewater Sediments

Autoři

  • Kavyaa Venkat Department of Biotechnology, M.S. Ramaiah Institute of Technology, Bangalore, India
  • Jyoti Kumari Centre for Nanobiotechnology, VIT University, Vellore, India
  • Chandraprabha M. N. Department of Biotechnology, M.S. Ramaiah Institute of Technology, Bangalore, India
  • Amitava Mukherjee 2Centre for Nanobiotechnology, VIT University, Vellore, India

Klíčová slova:

titanium dioxide nanoparticles, cytotoxicity, ROS

Abstrakt

 Studies suggest that approximately 4.6 million ton of pigmentary titanium dioxide (TiO2) is consumed annually worldwide. Owing to the increased industrial and consumer applications, there is growing concern regarding the possible risk associated with their environmental exposure. TiO2 dust, when inhaled, has been classified by the International Agency for Research on Cancer (IARC) as an IARC Group 2B carcinogen, meaning it is possibly carcinogenic to humans. TiO2 nanoparticles (NPs) can enter the aquatic environment either through direct or indirect release as effluents from nano-paints, sunscreen lotions, food additives, medical use, dismantling of batteries, etc. Therefore, the aim of the present study was to explore the difference in toxicity mechanism of TiO2 NPs at low concentrations (1 μg/ml), in a bacterial consortium consisting of Pseudomonas aeruginosa, Bacillus altitudinis and Bacillus tequilensis, isolated from lakewater sediments, under light and dark conditions. The cytotoxicity of the NPs was studied by performing several biochemical assays for the assessment of membrane permeability, cell viability, oxidative stress and secretion of exo-polysaccharides. It was found that there was generation of ROS, which may have resulted in the decrease in cell viability. Also, the cytotoxic effects were found to be more pronounced under dark than under light conditions.Keywords: titanium dioxide nanoparticles, cytotoxicity, ROS

Stažení

Publikováno

2014-04-28

Číslo

Vol 4 No 1 (2014)

Sekce

Research Articles

Licence

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

·         I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.

·         I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).

·         I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.

·          I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

_______________

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.