Garlic and Onion Extracts Enhance the Yield of T2 Bacteriophage in E. coli

Autoři

  • Gerald Goldstein Botany and Microbiology Department Ohio Wesleyan University
  • Owen L. Kelling
  • Gregory L. Serpa
  • Karli L. Sturgill
  • Madeleine M. Vroom

Abstrakt

It has been demonstrated that garlic and some of its components have anti-bacterial and/or anti-viral activity for viruses of humans in vitro.  Increasing concentrations of garlic extract have no effect on the replication of E. coli cultured in nutrient broth.  Increasing concentrations of garlic extract inhibit the replication of T4 Bacteriophage in E. coli but, surprisingly, E. coli cells treated with garlic extract produced a yield of T2 Bacteriophage up to 184% of the control culture.  Onion extract and allicin, a purified form of garlic, exhibit a similar stimulatory effect on the replication of T2 Bacteriophage.  The earlier garlic extract was added to the E. coli cells before infection, the greater was the enhancement of the T2 Bacteriophage yield.  Treatment of E. coli cells with garlic extract has no effect on the adsorption of T2 Bacteriophage to E. coli cells.  Increasing amounts of glucose added to E. coli cultures infected with T2 Bacteriophage caused an increase in the yield of the Bacteriophage but increasing amounts of glucose added to E. coli cultures treated with garlic extract and then infected with T2 Bacteriophage caused a decrease in the Bacteriophage yield. These results suggest that garlic extract stimulation of T2 Bacteriophage yield is due to the stimulation of cAMP production by E. coli cells.   Keywords: Garlic extract, Onion extract, T2 Bacteriophage, cAMPCite this Article Gerald Goldstein, Owen L. Kelling, Gregory L. Serpa, leine et al. Garlic and Onion Extracts Enhance the Yield of T2 Bacteriophage in E. coli. Research & Reviews: A Journal of Biotechnology. 2015; 5(2): 8–16p.

Biografie autora

  • Gerald Goldstein, Botany and Microbiology Department Ohio Wesleyan University
    Professor of MicrobiologyBotany and Microbiology Department

Stažení

Publikováno

2015-07-29

Číslo

Vol 5 No 2 (2015)

Sekce

Research Articles

Licence

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

·         I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.

·         I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).

·         I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.

·          I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

_______________

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.