Study on Efflux Pump Inhibition Activity of Marine Actinomycetes against Oflaxcin Resistsnt M. tuberculosis

Forfattere

  • Gajalakshmi P DEPARTMENT OF MICROBIOLOGY, DHANALAKSHMI SRINIVASAN COLLEGE OF ARTS AND SCIENCE FOR WOMEN PERAMBALUR 621212 TAMIL NADU INDIA
  • Raja A
  • Arthur James R

Resumé

Studies were conducted to isolate marine actinomycetes active against oflaxcin resistant M. tuberculosis. Out of 25 actinomycetes isolates designated as ECRMP 4 showed potent antimycobacterial activity against all three tested Mycobacterial strains. Representative bioactive isolate was characterized using phenotypic property and identified as Micropolyspora sp. The zone of inhibition was 20 ± 0.1 mm (62% RIZD) against M. tuberculosis and 18 ± 0.6 mm (100% RIZD) against OFLR M. tuberculosis. The bioassay of crude compound prevails that the Rf value of compound is 0.16. In this study we evaluated the role of the efflux pumps in quinolone resistance by using carbonyl cyanide m-chlorophenyl hydrazone (CCCP), 2,4-dinitrophenol (DNP) and verapamil against clinical isolate of M. tuberculosis. The MIC levels of OFL showed twofold reductions in presence of CCCP, verapamil and DNP while in case of ECRMP 4 Micropolyspora sp. metabolite showed fourfold reductions in MIC. These finding suggest the involvement of active efflux pumps activity of isolated compound from Micropolyspora can be able to produce noval antimycobacterial molecules. Keywords: Micropolyspora, oflaxcin, relative zone of inhibition, efflux pump

Forfatterbiografi

  • Gajalakshmi P, DEPARTMENT OF MICROBIOLOGY, DHANALAKSHMI SRINIVASAN COLLEGE OF ARTS AND SCIENCE FOR WOMEN PERAMBALUR 621212 TAMIL NADU INDIA
    assistant professordepartment of microbiology

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Publiceret

2014-09-26

Nummer

Årg. 4 Nr. 2 (2014)

Sektion

Research Articles

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