Production of Xylanase on Different Synthetic Media by Three Fruit-Rot Fungi of Banana (Musa paradisiaca L.)

Autor/innen

  • Supriya Sarkar kakatiya university Warangal Andhra Pradesh

Schlagwörter:

Xylanase, banana, fruit-rot fungi, media, carboxymethyl cellulose

Abstract

Production of xylanase by three fruit-rot fungi – Macrophomina phaseolina R-4242, Fusarium oxysporum sp.QJC-1403 and Nigrospora oryzae NRRL 54030 – isolated from the decaying fruits of banana on (Musa paradisiaca L.) on seven different synthetic media was investigated. The results showed that all the three pathogens studied were able to produce xylanase; however, the enzyme activity varied with the substratum and the pathogen inoculated. Medium G (xylose-peptone medium) supported good growth and maximum xylanase production (166.2 U/ml) by M. phaseolina, while F. oxysporum and N. oryzae recorded maximum xylanase production (154.1 U/ml and 167.3 U/ml) in medium B [Asthana and Hawker’s medium supplemented with 0.5% of carboxymethyl cellulose (CMC)]. Supplementation of production media with external carbon source CMC had positive effect on enzyme production by F. oxysporum and N.oryzae but M. phaseolina exhibited less enzyme activity in the presence of CMC, suggesting the constitutive nature of the enzyme produced by it. The study of period of incubation for xylanase production was optimized. Xylanase production was found to be optimum on 10th day of incubation period.

Autor/innen-Biografie

  • Supriya Sarkar, kakatiya university Warangal Andhra Pradesh
    Dept.of Microbiology

Downloads

Veröffentlicht

2012-01-18

Ausgabe

Bd. 1 Nr. 3 (2011)

Rubrik

Research Articles

Lizenz

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

·         I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.

·         I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).

·         I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.

·          I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

_______________

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.