L-glutaminase of Vibrio sp. from White-Spot Virus disease out-broken shrimp pond

Авторы

  • Jeya Prakash Research Scholar, CAS in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, South India
  • Poorani Eswaramoorthi Assistant Professor, Department of Biotechnology, Anna University, Coimbatore – 18 Tamil Nadu
  • Anantharaman Raman Reader, CAS in Marine Biology, Annamalai University, Porto Novo – 608 502. Tamil Nadu

Ключевые слова:

partial purification, Penaeus monodon pond, white-spot virus, Vibrio sp

Аннотация

Bacteria from White Spot Viral (WSV) disease out-broken shrimp (Penaeus monodon) pond sediments located in the bank of Vellar estuary, South east coast of India were enumerated, enriched, screened by plate assay and confirmed as Vibrio sp. through biochemical tests. Extracellular L-glutaminase was eluted from Vibrio sp. by centrifugation and purified by downstream process. The final yield was 1.7% with 15.4 IU mg-1 of specific activities, which was 17.2 fold purified. The eluted L-glutaminase was found as heterodimer (28,267 kDa and 17,391 kDa) due to the symbiotic relationship between Vibrio sp. and WSV.

Биографии авторов

  • Jeya Prakash, Research Scholar, CAS in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, South India
    C/o Dr.P.AnanatharamanReader,Department of Marine Microbiology and Biofuels
  • Poorani Eswaramoorthi, Assistant Professor, Department of Biotechnology, Anna University, Coimbatore – 18 Tamil Nadu
    Assistant Professor, Department of Biotechnology, Anna University, Coimbatore – 18
  • Anantharaman Raman, Reader, CAS in Marine Biology, Annamalai University, Porto Novo – 608 502. Tamil Nadu
    Reader, CAS in Marine Biology, Annamalai University,Porto Novo – 608 502. Tamil Nadu, South India.

Загрузки

Опубликован

2011-02-23

Выпуск

Том 1 № 1 (2011)

Раздел

Research Articles

Лицензия

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