Cell Membrane Immunogens of a Biofilm-producing Staphylococcus aureus Isolate Adjuvanted with Bacterial Chromosomal DNA Are Protective in a Mouse Mastitis Model

Autores

  • Krishan Sharma Immunology Section, Department of veterinary Microbiology, Lala Lajpat Rai University of veterinary & Animal Sciences, Hisar-125004 (Haryana)
  • Ajit Singh Professor & Head Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary & Animal Sciences

Palavras-chave:

Staphylococcus aureus, biofilms, cell membrane antigens, chromosomal DNA adjuvant, murine mastitis model

Resumo

Biofilm-forming staphylococci have been implicated in persistent bovine udder infections. Prophylactic potential of Staphylococcus aureus biofilm phenotype-associated immunogens in mouse models of systemic, but not intramammary infections has been demonstrated. In the present study, the authors observed protection in a murine model of intramammary infection by two subcutaneous injections at four-week interval of biofilm-forming S. aureus cell membrane antigens mixed with homologous chromosomal DNA as adjuvant, when challenged after eight weeks by intramammary route with 106 CFUs of biofilm-forming S. aureus. The immunoblot analysis indicated that five biofilm phenotype-associated polypeptides, 105, 90, 46, 36, and 33 kDa were more important than others as immunogens. These findings would be useful in selection of biofilm phenotype-associated S. aureus immunogen(s) for a candidate vaccine against bovine mastitis.

Biografias do Autor

  • Krishan Sharma, Immunology Section, Department of veterinary Microbiology, Lala Lajpat Rai University of veterinary & Animal Sciences, Hisar-125004 (Haryana)
    Presently: Veterinary Surgeon(HVS-I), Gatauli, Distt. Jind (Haryana)
  • Ajit Singh, Professor & Head Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary & Animal Sciences
    as above

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Publicado

2014-01-02

Edição

Vol. 3 N.º 3 (2013)

Secção

Research Articles

Licença

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