Isolation and Characterization of Protease Producing Organism Bacillus Species from Slaughter House Waste

Autoři

  • ANTONY SUNDHRAN Research Scholar, Manonmaniam Sundaranar University,Sri paramakalyani Centre of Excellence in Environmental Science, Alwarkurichi 627412
  • S Laju

Klíčová slova:

Slaughter house, protease enzyme, column chromatography, Bacillus sp.

Abstrakt

The purpose of this investigation was to isolate protease producing bacteria from slaughter house waste collected in local area of Kaliakkavilai. The Bacillus sp. was identified by several biochemical and physiological tests. The effect of different production parameters such as pH, temperature, metal ions concentration and substrate concentration for protease production by the isolated bacterial strain were studied. The enzyme production was assayed in submerged fermentation with optimized parameters to produce maximum protease activity and specific activity was observed as 1.067 and 0.011 µmol/ml/min. The partial purification of protease was done by column chromatography. Keywords: Slaughter house, protease enzyme, column chromatography, Bacillus sp.

Stažení

Další soubory

Publikováno

2014-01-01

Číslo

Vol 3 No 3 (2013)

Sekce

Research Articles

Licence

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

·         I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.

·         I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).

·         I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.

·          I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

_______________

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.