Increased Erythrocyte Osmotic Fragility and Serum Interleukin Eight Titers are Hallmarks of Patients with Dengue Virus Infection

Autori

  • Patra G Department of Laboratory Medicine, Calcutta School of Tropical Medicine, 108, C.R.Avenue, Kolkata 700073, India
  • Naskar A
  • Bhattacharya R.S
  • Mukherjee A
  • Ray Y
  • Bandyopadhyay B
  • Biswas A.K
  • Saha B
  • Mukhopadhyay S

Parole chiave:

Dengue fever, Erythrocytes, Interferon-γ, Interleukin-8, Osmotic fragility

Abstract

To investigate serum IFN-γ and IL8 titers, osmotic fragility and membrane cholesterol, and protein composition of erythrocytes of dengue fever patients. Seventy seven confirmed dengue patients along with 69 patients with other febrile infection (OFI) and 77 healthy controls (HC) were included in the study. The osmotic fragility of red blood cells was monitored using different concentrations of sodium chloride. The quantification of serum IFN-γ and IL-8 was carried out using commercial ELISA kits at baseline (early phase) and after 7 days of the infection (late phase). The levels of cholesterol and total protein were assessed in red blood cell membrane. Statistical analysis was performed using statistical software. Significant differences were found between erythrocyte mean corpuscular fragility (MCF) of dengue patients vs. healthy control (p < 0.001). Considerable correlation (r = 0.8949, p < 0.0001) was established between cholesterol to protein ratio of erythrocyte membrane vs. MCF values. Significant differences in serum IFN-γ and IL-8 titers of dengue patients were obtained at early and late phase of the infection. A significant positive correlation had also been established between erythrocyte MCF values and serum IL-8 titers of dengue patients (p < 0.0001, r = 0.9288). Mean corpuscular fragility and serum IL-8 titers are important clinical laboratory parameters for monitoring dengue infection progression.Keywords: dengue fever, erythrocytes, interferon-γ, interleukin-8, osmotic fragility.

Biografia autore

  • Patra G, Department of Laboratory Medicine, Calcutta School of Tropical Medicine, 108, C.R.Avenue, Kolkata 700073, India
    FacultyDepartment of Laboratory Medicine,Calcutta School of Tropical Medicine,108, C.R.Avenue, Kolkata 700073, India

Dowloads

File supplementari

Pubblicato

2014-08-12

Fascicolo

V. 4 N. 2 (2014)

Sezione

Research Articles

Licenza

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

·         I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.

·         I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).

·         I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.

·          I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

_______________

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.