Determination of Gatifloxacin in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry

Forfattere

  • Rajendra Prasad Assistant Professor, PG Department of Chemistry, JSS College of Arts, Commerce & Science (Affiliated to University of Mysore), Ooty Road, Mysore-570025, India
  • N. Rajendraprasad
  • K. Basavaiah

Resumé

Gatifloxacin (GTF) is an antibiotic of the fourth-generation fluoroquinolone family that inhibits the bacterial enzymes DNA gyrase and topoisomerase IV. A reliable, sensitive and selective isocratic reversed phase liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed to determine gatifloxacin (GTF) in human urine. GTF, in urine after solid-phase extraction with SPE cartridge, was analyzed on a C18 column (Symmetric 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of 5 mM ammonium formate (pH 2.9):acetonitrile (20:80, v/v). A linear relationship was obtained over a concentration range of 10–1000 ng mL-1 GTF with the lower limit of quantitation value of 10 ng mL-1. The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was <11.1%. The accuracy determined at three different concentrations of GTF (25.0, 500.0 and 850.0 ng mL-1) was within 1%.Keywords: Gatifloxacin, quantification, liquid chromatography-tandem mass spectrometryCite this Article:Anil Kumar UR, Rajendraprasad N, Basavaiah K. Determination of Gatifloxacin in Human Urine by Liquid Chromatography-Tandem Mass Spectrometry. Research & Reviews: A Journal of Pharmaceutical Science. 2015; 6(1): 1–6p.

Forfatterbiografi

  • Rajendra Prasad, Assistant Professor, PG Department of Chemistry, JSS College of Arts, Commerce & Science (Affiliated to University of Mysore), Ooty Road, Mysore-570025, India
    ChemistryAssistant Professor

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2015-05-01

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Årg. 6 Nr. 1 (2015)

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Research Articles

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