Development and Validation of Liquid Chromatography- Tandem Mass Spectrometric Method for Assay of Zidovudine in Human Urine

著者

  • Rajendra Prasad Assistant Professor, PG Department of Chemistry, JSS College of Arts, Commerce & Science (Affiliated to University of Mysore), Ooty Road, Mysore-570025, India
  • Kanakapura Basavaiah
  • Nagaraju Rajendraprasad

キーワード:

Zidovudine、 Quantification、 Liquid Chromatography-Tandem Mass Spectrometry、 Human urine、 Determination

要旨

A sensitive and selective liquid chromatographic-tandem mass spectrometric (LC-MSMS)method was developed to determine zidovudine (ZDV) in human urine. After solidphaseextraction with SPE cartridge, the urine sample was analyzed on a C18 column(Symmetry 3.5 μm; 75×4.6 mm i.d) interfaced with a triple quadrupole tandem massspectrometer. Positive electrospray ionization was employed as the ionization source. Themobile phase consisted of water-acetonitrile (20:80, v/v).The method was linear over aconcentration range of 10–1000 ng mL-1. The lower limit of quantitation was 10 ng mL-1.The intra-day and inter-day relative standard deviation across three validation runs overthe entire concentration range was <9.5%. The accuracy determined at threeconcentrations (150, 350 and 750 ng mL-1 ZDV) was within ±0.73% in terms of relativeerrors.Keywords: Zidovudine, quantification, liquid chromatography-tandem massspectrometry, human urine, determination

著者略歴

  • Rajendra Prasad、 Assistant Professor, PG Department of Chemistry, JSS College of Arts, Commerce & Science (Affiliated to University of Mysore), Ooty Road, Mysore-570025, India
    ChemistryAssistant Professor

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発行日

2015-01-21

巻号

巻 5 号 3 (2014)

セクション

Research Articles

ライセンス

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