Arsenic Induced Cytotoxicity Study Against Human Epithelial Carcinoma (HeLa) Cell

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  • Md. Shariful Islam Department of Animal Husbandry and Veterinary Science, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Alam Khan Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh Department of Metabolism and Aging, The Scripps Research Institute, Florida-33458, USA
  • Hrithita Aftab Department of Material Science and Engineering, University of Rajshahi, Rajshahi-6205, Bangladesh
  • Yoshifumi Kusumoto International University of Kagoshima, 8-31-1 Seiryo, Kagoshima 890-0032, Japan

Klíčová slova:

Arsenite, HeLa Cells, Toxicity, Cell viability, Trypan blue assay, Hematocytometer

Abstrakt

The present investigation was undertaken to find out the cytotoxic effects of various amounts of arsenic exposure to human epithelial carcinoma (HeLa) cell. Cells were treated with a final concentration (0, 10, 20, 30, 40 and 50 µg/l) of arsenite in 5 ml MEM for 24 h where 0 concentration was considered as control. Cell viability was determined by the trypan blue (TB) exclusion test using hematocytometer and automated BIO-RAD TC 10TM cell counting machine. It was demonstrated that survival rate of cancer cells treated with arsenite (0, 10, 20, 30, 40 and 50 µg/l) for 24 h decreased in a dose-dependent manner. Further, it was revealed that arsenite caused a time-dependent increase in cell death. In the highest concentration of arsenite (50 µg/l) cell survival rate was 1% and 2% in manual and automated counting. The possible mechanism that promote cytotoxicity of arsenite was addressed as oxidative damage of cells.  Keywords: Arsenite, HeLa Cells, Toxicity, Cell viability, Trypan blue assay, HematocytometerCite this Article  Islam S, Khan A, Aftab H, Kusumoto Y. Arsenic Induced Cytotoxicity Study against Human Epithelial Carcinoma (HeLa) Cell. Research and Reviews: A Journal of Toxicology (RRJoT). 2015; 5(1): 1–8p.

Biografie autora

  • Md. Shariful Islam, Department of Animal Husbandry and Veterinary Science, Faculty of Agriculture, University of Rajshahi, Rajshahi-6205, Bangladesh

Stažení

Publikováno

2015-04-07

Číslo

Vol 5 No 1 (2015)

Sekce

Review Articles

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