Engineering Fatty Acid Oxidation Pathway for the Production of Yeast-based Monomers

Abstract

Yeast-based monomers have a wider application in many fields and used for polymer production. Yeast system is used to improve the production of such valuable monomers by exploiting the metabolic pathways of interest. Alkane-utilizing yeasts mediate the fatty acid catabolism inside the peroxisomes primarily through beta (β) oxidation pathway, which breaks up the long chain fatty acids resulting in the synthesis of energy producing molecules. This allows the reduced inflow of the fatty acid substrate to the secondary pathway, omega (ω) oxidation resulting in lower productivity of their metabolites, which are of commercial importance. Therefore strategies are developed to bring up their enhanced production by nullifying the expression of the genes which are responsible for the primary pathway of fatty acid oxidation. It can be achieved by the construction of gene-disruption cassettes. This review article has its focus on engineering the yeast strain for the production of monomers.