Identification of Bacterial Strain from Waste Decay Material Using 16s rRNA Ribotyping

Abstract

Ribotyping is a classical technique in molecular studies where conserved and unique DNA sequences, more specifically 16s RNA, are investigated to differentiate among strains of microorganisms. This method was utilized in the current study to identify the strains of bacteria present in waste decaying matter. Waste material was collected from Indian Institute of Technology (IIT), Delhi campus, Hauz Khas, New Delhi, and cultured for bacterial growth. Purified bacterial cultures were used to isolated genomic DNA which was subsequently used for PCR based amplification of 16s RNA gene. Sequencing and computational analysis of the generated sequences revealed the presence of two bacterial strains Bacillus pumulis strain IK-MB13-518F and B. licheniformis strain MAC-110.

Keywords: Ribotyping, decay waste, Bacillus pumulis, B. licheniformis

Cite this Article

Suresh Kumar, Bhawna Garg, Bhardwaj Ankur R, et al. Identification of Bacterial Strain from Waste Decay Material Using 16s rRNA Ribotyping. Research & Reviews: A Journal of Life Sciences. 2016; 6(3): 1–4p.